Elucidation of NO reduction mechanism in soluble NO reductase by time-resolved crystallography with photosensitive caged compound
نویسندگان
چکیده
منابع مشابه
Proton delivery in NO reduction by fungal nitric-oxide reductase. Cryogenic crystallography, spectroscopy, and kinetics of ferric-NO complexes of wild-type and mutant enzymes.
Fungal nitric-oxide reductase (NOR) is a heme enzyme that catalyzes the reduction of NO to N(2)O through its ferric-NO complex, the first intermediate of the catalysis. Crystal structures of the ferric-NO forms of wild type (WT) fungal NOR, and of the Ser(286) --> Val and Ser(286) --> Thr mutant enzymes were determined to 1.7-A resolution at cryogenic temperature (100 K). This shows a slightly ...
متن کاملMechanism of the nucleotidyl-transfer reaction in DNA polymerase revealed by time-resolved protein crystallography
Nucleotidyl-transfer reaction catalyzed by DNA polymerase is a fundamental enzymatic reaction for DNA synthesis. Until now, a number of structural and kinetic studies on DNA polymerases have proposed a two-metalion mechanism of the nucleotidyl-transfer reaction. However, the actual reaction process has never been visualized. Recently, we have followed the nucleotidyl-transfer reaction process b...
متن کاملSelective Catalytic Reduction of CuO/SiO2 Nano-composites towards NO Reduction in Gas-phase
The xerogel samples were prepared by hydrolysis and condensation of tetraethyl orthosilicate (TEOS) by the sol-gel method. In this investigation, a new molar ratio of H2O/TEOS was determined to be 11.7. Also, the necessary amounts of tri-hydrated copper nitrate and penta-hydrated copper sulfate were added to the solution in such a manner that the concentration of the copper oxide in final solut...
متن کاملAnalysis of the HindIII-catalyzed reaction by time-resolved crystallography
In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII-DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions, indicated by relatively higher electron den...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2019
ISSN: 2053-2733
DOI: 10.1107/s2053273319095366